MARC details
| 000 -LEADER |
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| fixed length control field |
02381nam a22003733a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
UPMIN-00003300471 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
UPMIN |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240309152054.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
240309b |||||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
DLC |
| Transcribing agency |
UPMin |
| Modifying agency |
upmin |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
LG993.5 2008 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) |
F62 L84 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lumakin, Charles Luke Umbrosa. |
| Relator term |
author |
| 9 (RLIN) |
25415 |
| 245 #3 - TITLE STATEMENT |
|---|
| Title |
An Amylase-producing microorganism isolated from the ggut of sago palm weevil (Rhynchophorus spp.) larvae / |
| Statement of responsibility, etc. |
Charles Luke Umbrosa Lumakin. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Date of publication, distribution, etc. |
2008 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
49 leaves. |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2008 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
An amylase-producing microorganism was isolated from the gut of Rhynchophorus spp. larva (batud) found inside the infested trunk of sago (Metroxylon sagu Rottb.). the microorganism henceforth we call isolate 2, is Gram positive, single rods. The colony was small (about 1 mm diameter), white and transparent, entire, with a smooth texture having a convex elevation. Out of 13 microorganisms screened by their starch-clearing ability on starch agar plate, Isolate 2 was observed to be the highest amylase producing microorganism based on enzyme assay. The enzyme production was further improved by cultivating the isolate in MRS medium supplemented with raw sago starch for 18 hours without CaCO3. The crude enzyme obtained was partially purified in 2 stages, namely: ammonium sulphate precipitation coupled with dialysis, and ion-exchange chromatography using DEAE-Cellulose. Partial purification yielded an amylolytic enzyme with a specific activity of 43.72 U/mg protein at 2.15 fold purification and 34.76% recovery. The significance of this study lies in the potential of this enzyme to reduce the cost of processing starch into glucose if proven later to be a raw starch digestive amylase. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Sago. |
| 9 (RLIN) |
25255 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Amylase. |
| 9 (RLIN) |
25326 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enzyme assays. |
| 9 (RLIN) |
25416 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Alpha-amylases. |
| 9 (RLIN) |
25417 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enzyme purification. |
| 9 (RLIN) |
25418 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Termites. |
| 9 (RLIN) |
25419 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Insect amylase. |
| 9 (RLIN) |
25420 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Insect gut microorganisms. |
| 9 (RLIN) |
25421 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Sago palm weevils. |
| 9 (RLIN) |
25422 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Starch. |
| 9 (RLIN) |
1680 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Sago |
| General subdivision |
Commodities. |
| 9 (RLIN) |
25423 |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE |
|---|
| Main curriculum objective |
Undergraduate Thesis |
| Curriculum code |
FST200, |
| Source of term or code |
BSFT |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| a |
Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| a |
UP |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |
