Effect of osmotic potential of culture media on growth of sago palm (Metroxylon sagu Rottb.) explants in vitro / (Record no. 2458)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02169nam a22002533a 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00004810035 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20221005150637.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2010 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 C36 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Calamba, Kristine Joy B. |
| 245 ## - TITLE STATEMENT | |
| Title | Effect of osmotic potential of culture media on growth of sago palm (Metroxylon sagu Rottb.) explants in vitro / |
| Statement of responsibility, etc. | Kristine Joy B. Calamba |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2010 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 52 leaves. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2010 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In vitro propagation of sago palm is necessary due to its high economic importance. The optimization of media is one of the most important steps in the establishment of in vitro plant culture systems. Sugars as major source of carbon also act as good osmoticum in culture media. Since there is no research work conducted on their effect on sago palm, this study was conducted to evaluate the influence of various concentrations of sucrose as osmotic regulator on the growth of sago palm explants and to initiate meristematic shoots on explants. Sago palm shoot apical meristem was used as explant and inoculated in half-strength and full-strength modified Murashige and Skoog (MMS) media with sucrose concentration of 0.0 M (control), 0.1 M, 0.2 M, 0.3 M, and 0.4 M. Preliminary experiment showed lower survivability of explants after four weeks of incubation because of contamination. Upon using a new sterilization protocol, higher survivability rate was observed and signs of growth were noted. Explants favored the MS media containing 0.2 M sucrose with osmotic potential -0.507 MPa because of their high increase in weight. Higher osmotic potential was observed to have brown explants after four weeks. However, statistical analysis showed no significant differences among the treatments. Therefore, more study is required to further verify the effects of sucrose and perhaps other osmotic agents on sago palm |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Sago palm |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Osmoticum |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Osmotic potential |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | In vitro culture |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-10-05 | UAR-T-gd1519 | LG993.5 2010 B4 C36 | 3UPML00033212 | 2022-10-05 | 2022-10-05 | Thesis | ||||||
| University Library | University Library | 2022-10-05 | CSM-T-gd2259 | LG993.5 2010 B4 C36 | 3UPML00012557 | 2022-10-05 | Thesis |