MARC details
| 000 -LEADER |
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| fixed length control field |
02170nam a22002893a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
UPMIN-00005017247 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
UPMIN |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240228160451.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
240228b |||||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
DLC |
| Transcribing agency |
UPMin |
| Modifying agency |
upmin |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
LG993.5 2010 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) |
F62 G86 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Gumia, Robelyn Pieza. |
| Relator term |
author |
| 9 (RLIN) |
25325 |
| 245 ## - TITLE STATEMENT |
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| Title |
Isolation and partial characterization of a potential amylolytic microorganism from decomposing sago palm pith / |
| Statement of responsibility, etc. |
Rubelyn Pieza Gumia. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Date of publication, distribution, etc. |
2010 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
55 leaves |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2010 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Sixty-seven potentially amylolytic microorganisms were isolated from decomposing sago palm pith. Twenty-five starch hydrolyzing isolates were purified and screened for amylolytic activity. SB125, the most promising isolate, had an amylolytic activity of 1.45 U/mL. Morphological biochemical characterization of SB 125 revealed that it is a coccus, facultatively aerobic and Gram-negative. It is tested negative for the catalase test, for indole production and litmus milk reaction. However, it was positive for methyl red staining and starch hydrolysis. Amylase activity increased when SB 125 was grown on modified de Mann, Rogosa and Sharpe (MRS) medium without Ca2+ supplementation, under shaking condition, Partial enzyme purification yielded a specific activity of 5.51 U/mg at 1.32 fold purification and 13.4% recovery. The partially purified enzyme had an optimum activity at 400C and pH 8.5. enzyme immobilized by surface adsorption yielded a specific activity of 11.87 U/mg, significantly higher than the unimmobilized enzyme which yielded 5.51 U/mg. Enzyme immobilized by entrapment showed a decrease in activity at 3.65 U/mg, indicating that surface adsorption is more promising if SB 125 were to be developed for industrial application. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Amylase |
| 9 (RLIN) |
25326 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Rotting sago palm pith |
| 9 (RLIN) |
25327 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enzyme characterization |
| 9 (RLIN) |
1674 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enzyme immobilization |
| 9 (RLIN) |
25328 |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE |
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| Main curriculum objective |
Undergraduate Thesis |
| Curriculum code |
FST200, |
| Source of term or code |
BSFT |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| a |
Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| a |
UP |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |
