DNA methylation profiling of juvenile spiny and nonspiny sago palm (metroxylon sagu Rottb.) using high performance liquid chromatography analysis (Record no. 2595)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01928nam a22002893a 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00005875628 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20221005151937.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | UP |
| Modifying agency | dupmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG 993.5 2011 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 M33 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mabras, Maria Brigida C. |
| 245 ## - TITLE STATEMENT | |
| Title | DNA methylation profiling of juvenile spiny and nonspiny sago palm (metroxylon sagu Rottb.) using high performance liquid chromatography analysis |
| Statement of responsibility, etc. | Maria Brigida C. Mabras. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2011 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 68 leaves. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2011 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Several studies have shown that phenotypic variations in sago palm such as armature (presence or absence of spines) are not associated with genetic variation. It is hypothesized that epigentic modification such as DNA methylation might be correlated with armature in sago palm. This hypothesis was investigated in this study with the aims to determine the biological role of DNA methylation and to verify if the relationship between transcription and DNA methylation profiles of sago palm through HPLC analysis. Thus included optimization of HPLC conditions for better resolution of peaks which further aid in detecting the peaks of dC and 5mdC in the DNA samples analyzed. Results showed that the wavelength of 280 nm used to monitor effluents efficiently distinguished signal from noise, while flow rate 0.2 mL/min resulted to peaks more distinguishable form each other. HPLC analysis of DNA methylation showed 5mdC was 51.9084% for spiny juvenile while 51.917% for non-spiny juvenile sago palms. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Metroxylon sagu |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Epigenetic. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA methylation. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HPLC (High Performance Liquid Chromatography) |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Sago palm. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Spiny sago palm. |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Non-spiny sago palm. |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-10-05 | UAR-T-gd1894 | LG 993.5 2011 B4 M33 | 3UPML00033445 | 2022-10-05 | 2022-10-05 | Thesis | ||||||
| University Library | University Library | 2022-10-05 | CSM-T-gd2814 | LG 993.5 2011 B4 M33 | 3UPML00012749 | 2022-10-05 | 2022-10-05 | Thesis |