Extraction and partial purification of proanthocyanidins (PAC) from Cacao seeds / (Record no. 2603)

MARC details
000 -LEADER
fixed length control field 02182nam a22002893a 4500
001 - CONTROL NUMBER
control field UPMIN-00005875637
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20221005151937.0
040 ## - CATALOGING SOURCE
Original cataloging agency DLC
Transcribing agency UP
Modifying agency upmin
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) LG 993.5 2011
Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) F62 S56
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Simogan, Jerry Jr. Parparan.
245 ## - TITLE STATEMENT
Title Extraction and partial purification of proanthocyanidins (PAC) from Cacao seeds /
Statement of responsibility, etc. Jerry Parparan Simogan Jr.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Date of publication, distribution, etc. 2011
300 ## - PHYSICAL DESCRIPTION
Extent 44 leaves.
502 ## - DISSERTATION NOTE
Dissertation note Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2011
520 ## - SUMMARY, ETC.
Summary, etc. Extraction from fresh cacao seeds was carried out using 25%, 50%, 70%, and 95%n ethanol to determine what concentration gives the highest yield in extracting proanhocyanidins (PAC). The trend showed that as ethanol concentration increased, the yield also increased (25%=20.67%, 50%-50.80%, 70%-58.47% and 95%=80.20% of the entire PAC content of cacao seeds). The 95% EtOH, which yielded the highest, was then used for assessing efficiency of extraction protocol employed. The triple extraction test showed that single extraction is enough to extract majority of the PAC of the cacao seed powder and that 95% EtOH is far better solvent than hot water to extract PAC. The 95% EtOH was used for extracting crude PAC which was purified alter using Diaion HP-20 resin chromatographic column. There was a total yield from the water and 10% EtOH washing of 38.519% monitored through OD 280 scanning, which constitutes the contaminants removed from the extract. Proanthocyanidins were eluted using 70% ethanol. The chromatographic procedure using the Diaion enriched the PAC content per unit absorbance at 280 nm (OD280) of the crude extract 1.62 fold; thus, a partially purified PAC extract was obtained. There was an excellent recovery of the PAC content of the crude extract and no loss was recorded after purification.
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Proanthocyanidins.
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Extraction.
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 95% ethanol.
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Purification by Dia-ion HP20
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cacao seeds.
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PAC (Proanthocynidins)
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Crude extract.
658 ## - INDEX TERM--CURRICULUM OBJECTIVE
Main curriculum objective Undergraduate Thesis
Curriculum code FST200,
Source of term or code BSFT
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
a Fi
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
a UP
Holdings
Withdrawn status Lost status Damaged status Status Collection Home library Current library Date acquired Accession Number Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
          University Library University Library 2022-10-05 UAR-T-gd1905   LG 993.5 2011 F62 S56 3UPML00033508 2022-10-05 2022-10-05 Thesis
          University Library University Library 2022-10-05 CSM-T-gd2795   LG 993.5 2011 F62 S56 3UPML00012813 2022-10-05 2022-10-05 Thesis
 
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