Characterization of hydrolytic activity of raw starch-digesting amylase (RSDA) from Saccharomycopsis bubodii 2066 on sago (Metroxylon sagu Rottb.) starch / (Record no. 2604)

MARC details
000 -LEADER
fixed length control field 02188nam a22003133a 4500
001 - CONTROL NUMBER
control field UPMIN-00005875638
003 - CONTROL NUMBER IDENTIFIER
control field UPMIN
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240227142841.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 230130b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency DLC
Transcribing agency UPMin
Modifying agency upmin
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) LG 993.5 2011
Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) F62 C38
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Caubalejo, Rey Jacutin.
9 (RLIN) 1750
Relator term author
245 ## - TITLE STATEMENT
Title Characterization of hydrolytic activity of raw starch-digesting amylase (RSDA) from Saccharomycopsis bubodii 2066 on sago (Metroxylon sagu Rottb.) starch /
Statement of responsibility, etc. Rey Jacutin Caubalejo.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Date of publication, distribution, etc. 2011
300 ## - PHYSICAL DESCRIPTION
Extent 76 leaves.
502 ## - DISSERTATION NOTE
Dissertation note Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2011
520 3# - SUMMARY, ETC.
Summary, etc. A raw starch digesting amylase (RSDA) was purified from Saccharomycopsis bubodii 2066 which was isolated from Philippine Bubod starter. The free enzyme (dialysate) had a specific activity of 3.91U mg-1. It was immobilized by encapsulation with calcium alginate. Specific activity of the immobilized enzyme decreased to 0.26 U mg-1. Hydrolitic activity was evaluated through degree and rate of hydrolysis by determining reducing sugar content as a function of time. The reaction mixtures contained 0.11 mg protein and 0.7 mg protein, for the free and immobilized enzymes, respectively. Degree of hydrolysis using free and immobilized enzyme was 0.11 and 0.03 mg reducing sugar mg-1 protein, respectively. Adsorbability onto raw sago starch was not observed in immobilized enzyme which explains the negative results. On the contrary, the free enzyme almost adsorbed completely. Operational stability in immobilized enzyme was assessed through binding efficiency and enzyme reusability. Binding efficiency was 70.75% efficiency. After reusing the bead for 4 cycles. it retained 18.24% of the original activity.
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RSDA (Raw Starch Digesting Amylase)
9 (RLIN) 1751
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Saccharomycopsis bubodii 2066
9 (RLIN) 1752
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Hydrolytic activity.
9 (RLIN) 1753
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Immobilized enzymes.
9 (RLIN) 1754
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Glucoamylase.
9 (RLIN) 1755
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Sago starch.
9 (RLIN) 1454
658 ## - INDEX TERM--CURRICULUM OBJECTIVE
Main curriculum objective Undergraduate Thesis
Curriculum code FST200,
Source of term or code BSFT
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
a Fi
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
a UP
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Thesis
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Status Collection Home library Current library Shelving location Date acquired Source of acquisition Accession Number Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
    Library of Congress Classification   Not For Loan Preservation Copy University Library University Library Archives and Records 2014-02-07 donation UAR-T-gd1906   LG 993.5 2011 F62 C38 3UPML00033519 2022-10-05 2022-10-05 Thesis
    Library of Congress Classification   Not For Loan Room-Use Only College of Science and Mathematics University Library Theses 2011-12-07 donation CSM-T-gd2796   LG 993.5 2011 F62 C38 3UPML00012804 2022-10-05 2022-10-05 Thesis
 
University of the Philippines Mindanao
The University Library, UP Mindanao, Mintal, Tugbok District, Davao City, Philippines
Email: library.upmindanao@up.edu.ph
Contact: (082)295-7025
Copyright @ 2022 | All Rights Reserved