MARC details
000 -LEADER |
fixed length control field |
02188nam a22003133a 4500 |
001 - CONTROL NUMBER |
control field |
UPMIN-00005875638 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
UPMIN |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20240227142841.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
230130b |||||||| |||| 00| 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
DLC |
Transcribing agency |
UPMin |
Modifying agency |
upmin |
041 ## - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
LG 993.5 2011 |
Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) |
F62 C38 |
100 ## - MAIN ENTRY--PERSONAL NAME |
Personal name |
Caubalejo, Rey Jacutin. |
9 (RLIN) |
1750 |
Relator term |
author |
245 ## - TITLE STATEMENT |
Title |
Characterization of hydrolytic activity of raw starch-digesting amylase (RSDA) from Saccharomycopsis bubodii 2066 on sago (Metroxylon sagu Rottb.) starch / |
Statement of responsibility, etc. |
Rey Jacutin Caubalejo. |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Date of publication, distribution, etc. |
2011 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
76 leaves. |
502 ## - DISSERTATION NOTE |
Dissertation note |
Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2011 |
520 3# - SUMMARY, ETC. |
Summary, etc. |
A raw starch digesting amylase (RSDA) was purified from Saccharomycopsis bubodii 2066 which was isolated from Philippine Bubod starter. The free enzyme (dialysate) had a specific activity of 3.91U mg-1. It was immobilized by encapsulation with calcium alginate. Specific activity of the immobilized enzyme decreased to 0.26 U mg-1. Hydrolitic activity was evaluated through degree and rate of hydrolysis by determining reducing sugar content as a function of time. The reaction mixtures contained 0.11 mg protein and 0.7 mg protein, for the free and immobilized enzymes, respectively. Degree of hydrolysis using free and immobilized enzyme was 0.11 and 0.03 mg reducing sugar mg-1 protein, respectively. Adsorbability onto raw sago starch was not observed in immobilized enzyme which explains the negative results. On the contrary, the free enzyme almost adsorbed completely. Operational stability in immobilized enzyme was assessed through binding efficiency and enzyme reusability. Binding efficiency was 70.75% efficiency. After reusing the bead for 4 cycles. it retained 18.24% of the original activity. |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
RSDA (Raw Starch Digesting Amylase) |
9 (RLIN) |
1751 |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Saccharomycopsis bubodii 2066 |
9 (RLIN) |
1752 |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Hydrolytic activity. |
9 (RLIN) |
1753 |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Immobilized enzymes. |
9 (RLIN) |
1754 |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Glucoamylase. |
9 (RLIN) |
1755 |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Sago starch. |
9 (RLIN) |
1454 |
658 ## - INDEX TERM--CURRICULUM OBJECTIVE |
Main curriculum objective |
Undergraduate Thesis |
Curriculum code |
FST200, |
Source of term or code |
BSFT |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
a |
Fi |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
a |
UP |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Library of Congress Classification |
Koha item type |
Thesis |