Approximation of microbial load of beef using resazurin dye reduction technique / (Record no. 280)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01570nam a2200205 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00000009225 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220921140845.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2000 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 M47 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mesias, Marianette Noelle B. |
| 245 00 - TITLE STATEMENT | |
| Title | Approximation of microbial load of beef using resazurin dye reduction technique / |
| Statement of responsibility, etc. | Marianette Noelle B. Mesias |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2000 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 25 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2000 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The validity of the dye reduction technique in determining the microbial load of beef was tested. Beef samples were swabbed using sterile template and cotton swab sticks and then dropped in a test tube containing 5 ml nutrient medium with 0.2 ml of 0.04% resazurin dye. The time of change from blue to lavender endpoint was noted. Simultaneous with this, the beef was swabbed and diluted in 5 ml peptone water. Dulutions of 10-4, and 10-6 were each plated in triplicate in nutrient agar. These were incubated at 37oC for 48 hours. Correlation regression analysis of the reduction time and total plate count gave a high correlation coefficient of -0.93. a regression equation of y=172.27-20.009x was obtained and a coefficient of determination of 86% proved that this can be used to predict the microbial load given the reduction time and vice versa. Using the formula, spoilage of beef as defined by International Standards can be detected within 12-13 minutes |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-09-21 | UAR-T-gd029 | LG993.5 2000 B4 M47 | 3UPML00020760 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
| University Library | University Library | 2022-09-21 | CSM-T-gd662 | LG993.5 2000 B4 M47 | 3UPML00010995 | 2022-09-21 | 2022-09-21 | Thesis |