Callus induction and plant regeneration of Nepenthes truncata Macf. / (Record no. 621)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02025nam a2200229 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00000014464 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220921142401.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2007 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 D44 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Delgado, Danica Riza V. |
| 245 00 - TITLE STATEMENT | |
| Title | Callus induction and plant regeneration of Nepenthes truncata Macf. / |
| Statement of responsibility, etc. | Danica Riza V. Delgado. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2007 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 71 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2007 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Callus induction and plant regeneration of Nepenthes truncate was undertaken to determine the appropriate combination of explant and plant growth regulator for callus induction and for the optimization of the culture medium for callus proliferation and regeneration. Three types of explants were obtained from five-month old axenic seedlings; leaf with pitcher, shoot tip, and nodal segments. These explants were tested in different concentrations of NAA (0.5, 1, 1.5 and 2 mg L-1. Among the explants, leaf proved to be the most suitable for callus induction in ½ strength MS medium and good callus formation was obtained from the combination of 0.5 mg L-1 NAA + 0.05 mg L-1 TDZ. Browning of callus after three to four months was the main constant. To alleviate the problem of browning. Two culture media, ½ strength B5 and ½ strength MS, and two antioxidants/adsorbents, ascorbic acid and activated charcoal were tested against the controls (1/2 strength MS) without antioxidants/adsorbents using leaf as explant. The antioxidants/adsorbents did not seem to control browning but ½ strength MS with activated charcoal regenerated shoots in some treatment s, with a maximum of 7.5 shoots per explant in the combination of 0.5 mg L-1 NAA + 0.05 mg L-1 TDZ. Peptone as an organic supplement for the growth of callus was also studied but its effect was not significant |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plant tissue. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plant tissue culture. |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-09-21 | UAR-T-gd986 | LG993.5 2007 B4 D44 | 3UPML00031023 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
| University Library | University Library | 2022-09-21 | CSM-T-gd1622 | LG993.5 2007 B4 D44 | 3UPML00011874 | 2022-09-21 | 2022-09-21 | Thesis |