Isolation and identification of fungi with potential for biological control of Asiatic corn borer, Ostrinia furnacalis (Guenee) / Mishelle P. Escrupolo

By: Material type: TextTextLanguage: English Publication details: 2002Description: 28 leavesSubject(s): Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2002 Summary: Six cornfields from COMVAL having different corn borer infestation level were randomly selected for collection of specimen. Most of the fungi isolated were from corn borers collected in areas with high level of infestation except for one, the km 94 isolate. The first batch collected corn borer larvae were placed in petri plates containing filter paper moistened with distilled water and incubated for seven days. The fungal growth observed from the insect?s body were isolated, purified, and cultured on a Potato Dextrose Agar (PDA) medium. Identification of the isolates was done using a dichotomous key by Raymundo et.al. (1991). Two of the five isolates were identified belonging to genus Rhizopus while the remaining three belong to genus Aspergillus. The second batch of collected corn borer larvae were reared up to the third generation but a bioassay was not performed due to some constraints. Bioassay of the fungal isolates against corn borer must be done to determine its potential for biological control of the host insect
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Item type Current library Collection Call number Status Date due Barcode
Thesis University Library Non-Circulation LG993.5 2002 B4 E82 (Browse shelf(Opens below)) Available 3UPML00020801
Thesis University Library Reference/Room-Use Only LG993.5 2002 B4 E82 (Browse shelf(Opens below)) Available 3UPML00010442

Thesis (BS Biology) -- University of the Philippines Mindanao, 2002

Six cornfields from COMVAL having different corn borer infestation level were randomly selected for collection of specimen. Most of the fungi isolated were from corn borers collected in areas with high level of infestation except for one, the km 94 isolate. The first batch collected corn borer larvae were placed in petri plates containing filter paper moistened with distilled water and incubated for seven days. The fungal growth observed from the insect?s body were isolated, purified, and cultured on a Potato Dextrose Agar (PDA) medium. Identification of the isolates was done using a dichotomous key by Raymundo et.al. (1991). Two of the five isolates were identified belonging to genus Rhizopus while the remaining three belong to genus Aspergillus. The second batch of collected corn borer larvae were reared up to the third generation but a bioassay was not performed due to some constraints. Bioassay of the fungal isolates against corn borer must be done to determine its potential for biological control of the host insect

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