Morphological and molecular characterization of fourteen geographical isolates of metarhizium anisopliae (metsch.) sorokin / Dannel Patrisha Grace H. Hiquiana.
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TextLanguage: English Publication details: 2009Description: 46 leavesSubject(s): Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2009 Summary: Morphological data on the colony growth shape and microspcopic appearance and conidia morphometrics were obtained for the fourteen Metarhizium anisopliae geographical isolates from oryctes rhinoceros host. In addition, its genetic diversity and relatedness were determined using the RAPD markers. No apparent differences were observed on the colony growth in vitro. The spore morphology of all the isolates had a relative uniformity being single-celled, green conidia, cylindrical and elongated to slightly ellipsoidal, with rounded ends. The spore length mean ranged from 11.46 to 12.42 m with the extremes measured from CRN and ALA respectively. The obtained data in morphology along with other biological information suggest that the isolates at hand belong to the M. anisopliae var. majus type. The 6 RAPD primers generated 25 RAPD markers and a total of 280 amplified bands. An 80% monomorphism was produced with 224 bands while 56 bands were polymorphic (20%). Molecular sizes arrayed from 190 to 1517 bp, the smallest-sized fragments generated by HAG and LA in primer B and the largest being monomorphic, which were yielded by primers STB-3 and STB-5. This high degree of monomorphism was reflective of their strong genetic similarity and could be largely due to their asexual nature. The calculated genetic similarity coefficients ranged from 0.85 to 1.00 with the smallest value from LA and CGN. The largest similarity was found between ALA and QUE and also between DRC, CAL, MACO and CMI. LA was the least similar among the isolates. Genetic relationships of the isolates obtained a varying bootstrap support values. The UPGMA topology produced three clusters with all nodes having bootstrap values of less than 50% collapsed. The first cluster composed of 11 isolates from CGN down to HAG and the second and third lineages were formed by IRRI and LUC respectively. No correlation was found on the samples? genetic similarity and relationships with respect to its geographical origin suggesting that the samples having slight genetic variability may have originated from the same ancestral population and was disseminated in association to its host
| Item type | Current library | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Thesis | University Library | Non-Circulation | LG993.5 2009 B4 H47 (Browse shelf(Opens below)) | Available | 3UPML00032492 | |
| Thesis | University Library | Reference/Room-Use Only | LG993.5 2009 B4 H47 (Browse shelf(Opens below)) | Available | 3UPML00012400 |
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Thesis (BS Biology) -- University of the Philippines Mindanao, 2009
Morphological data on the colony growth shape and microspcopic appearance and conidia morphometrics were obtained for the fourteen Metarhizium anisopliae geographical isolates from oryctes rhinoceros host. In addition, its genetic diversity and relatedness were determined using the RAPD markers. No apparent differences were observed on the colony growth in vitro. The spore morphology of all the isolates had a relative uniformity being single-celled, green conidia, cylindrical and elongated to slightly ellipsoidal, with rounded ends. The spore length mean ranged from 11.46 to 12.42 m with the extremes measured from CRN and ALA respectively. The obtained data in morphology along with other biological information suggest that the isolates at hand belong to the M. anisopliae var. majus type. The 6 RAPD primers generated 25 RAPD markers and a total of 280 amplified bands. An 80% monomorphism was produced with 224 bands while 56 bands were polymorphic (20%). Molecular sizes arrayed from 190 to 1517 bp, the smallest-sized fragments generated by HAG and LA in primer B and the largest being monomorphic, which were yielded by primers STB-3 and STB-5. This high degree of monomorphism was reflective of their strong genetic similarity and could be largely due to their asexual nature. The calculated genetic similarity coefficients ranged from 0.85 to 1.00 with the smallest value from LA and CGN. The largest similarity was found between ALA and QUE and also between DRC, CAL, MACO and CMI. LA was the least similar among the isolates. Genetic relationships of the isolates obtained a varying bootstrap support values. The UPGMA topology produced three clusters with all nodes having bootstrap values of less than 50% collapsed. The first cluster composed of 11 isolates from CGN down to HAG and the second and third lineages were formed by IRRI and LUC respectively. No correlation was found on the samples? genetic similarity and relationships with respect to its geographical origin suggesting that the samples having slight genetic variability may have originated from the same ancestral population and was disseminated in association to its host
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