Whole-cell immobilization of entercoccus faecium DMF78 by gel entrapment for the direct lactic acid production from sago starch / Sheba Mae Magbanua Duque.

By: Material type: TextTextLanguage: English Publication details: 2009Description: 50 leavesSubject(s): Dissertation note: Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2009 Abstract: Enterococcus faecium DMF78 is a novel amylolytic lactic acid bacterium that has the ability to directly convert starch into L(+)-lactic acid. To further improve the lactic acid production, this bacterium was immobilized using three matrices, namely: agar, alginate and K-carrageenan. Each matrix was optimized to maximize lactic acid production. As for agar, the effects of agar quality, NaCl and agar concentrations were investigated. For alginate, the effects of calcium chloride concentration, hardening time and alginate concentration were tested, and for k-carrageenan, the effects of potassium chloride concentration and extended curing time with calcium chloride were studied. The lactic acid production using the above matrices, as optimized, was compared and lactic acid produced was determined using high performance liquid chromatography (HPLC). Data showed that the use of 3% alginate, extruded drop wise on 0.2 M CaCl2, and cured for 1 hour was the most efficient immobilizing matrix for E. faecium DMF78. Tests for reducing sugar and residual starch and estimation of cell wash-out were also determined. A yield of 3.011 g/L lactic acid was observed using E. faecium DMF78 immobilized in alginate, after 24 hours fermentation of sago starch. This can also be expressed as lactic acid productivity of 0.125 g.L-1.h-1 and a lactic acid yield (conversion) of 25.42%.
List(s) this item appears in: BS Food Technology
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Thesis Thesis University Library Theses Room-Use Only LG993.5 2009 F62 D86 (Browse shelf(Opens below)) Available 3UPML00012386
Thesis Thesis University Library Archives and Records Non-Circulating LG993.5 2009 F62 D86 (Browse shelf(Opens below)) Preservation Copy 3UPML00032702

Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2009

Enterococcus faecium DMF78 is a novel amylolytic lactic acid bacterium that has the ability to directly convert starch into L(+)-lactic acid. To further improve the lactic acid production, this bacterium was immobilized using three matrices, namely: agar, alginate and K-carrageenan. Each matrix was optimized to maximize lactic acid production. As for agar, the effects of agar quality, NaCl and agar concentrations were investigated. For alginate, the effects of calcium chloride concentration, hardening time and alginate concentration were tested, and for k-carrageenan, the effects of potassium chloride concentration and extended curing time with calcium chloride were studied. The lactic acid production using the above matrices, as optimized, was compared and lactic acid produced was determined using high performance liquid chromatography (HPLC). Data showed that the use of 3% alginate, extruded drop wise on 0.2 M CaCl2, and cured for 1 hour was the most efficient immobilizing matrix for E. faecium DMF78. Tests for reducing sugar and residual starch and estimation of cell wash-out were also determined. A yield of 3.011 g/L lactic acid was observed using E. faecium DMF78 immobilized in alginate, after 24 hours fermentation of sago starch. This can also be expressed as lactic acid productivity of 0.125 g.L-1.h-1 and a lactic acid yield (conversion) of 25.42%.

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