Micropropagation of Vanda sanderiana ("Waling-waling") using root tips : I. Initial studies on the standardization of sterilization technique and selection of suitable culture media / Cathy F. Fernandez
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TextLanguage: English Publication details: 2000Description: 25 leavesSubject(s): Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2000 Summary: Root tips from mature and clonally-propagated V. senderiana were subjected to different Clorox treatments ranging from 5% for 15 and 30 minutes and 10 to 20% for 5-15 minutes. Data obtained showed that mature root tip explants may be sterilized using 10 to 20% clorox for 5 minutes. Tests confirmed that clonally-propagated explants neednot be sterilized. Three types of media were evaluated: namely the modified White?s, modified Vacin-Went and modified Murashige and Skoog. Explants cultured in Vacin-Went medium showed positive growth signs through elongation but results were not sufficient to conclude that it is most suitable for root tip culture of V. sanderiana
| Item type | Current library | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Thesis | University Library | Non-Circulation | LG993.5 2000 B4 F47 (Browse shelf(Opens below)) | Available | 3UPML00020728 | |
| Thesis | University Library | Reference/Room-Use Only | LG993.5 2000 B4 F47 (Browse shelf(Opens below)) | Available | 3UPML00010984 |
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Thesis (BS Biology) -- University of the Philippines Mindanao, 2000
Root tips from mature and clonally-propagated V. senderiana were subjected to different Clorox treatments ranging from 5% for 15 and 30 minutes and 10 to 20% for 5-15 minutes. Data obtained showed that mature root tip explants may be sterilized using 10 to 20% clorox for 5 minutes. Tests confirmed that clonally-propagated explants neednot be sterilized. Three types of media were evaluated: namely the modified White?s, modified Vacin-Went and modified Murashige and Skoog. Explants cultured in Vacin-Went medium showed positive growth signs through elongation but results were not sufficient to conclude that it is most suitable for root tip culture of V. sanderiana
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