Identification of Rhizobacterial Diazotrophs of the family Azotobacteraceae from the sago palm (Metroxylon sagu Rottb.) / Maria Catherine B. Otero
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TextLanguage: English Publication details: 2005Description: 50 leavesSubject(s): Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2005 Summary: Three bacterial isolates were obtained from soil samples taken from Simulao, Bunawan (SI), a ricefield water-logged for most of the year, and Pulangpula, Trento (PL), found 1.3m from the bank of a freshwater stream. Azotobacter and Azomonas-specific medium and Beijerinckia-specific medium were used. Growth of the isolates was poorer on Beijerinckia-specific medium compared to that on Azotobacter and Azomonas-specific medium. Using conventional microbiological methods, the identity of each isolate was determined. PL-A1, identified to be Azotobacter, were Gram-positive, motile, aerobic, and catalase coccoids ranging from 1.5m to 2.5m in diametrr. Cysts formed ranged from 1.5m to 3.0m in diameter. Colonies formed were cream in color, circular, entire, convex, mucoid, and glistening, with diameter ranging from 0.01 mm to 0.16 mm. PL-A2, identified as Beijerinckia, were Gram-negative. Motile, aerobic, and catalase-positive slightly curved rods with the dimensions 0.5-1.5 m x 1.5-2.5 m. colonies formed were transparent, entire, convex, mucoidal and slimy measuring 0.08 mm to 0.51 mm in diameter. SI-B, identified as Azomonas, were Gram-negative, motile, aerobic, and catalese-positive coccoids ranging from 1.5 m to 3.0 m in diameter. Colonies formed were cream in color, circular, entire, convex, mucoid and glistening, with diameter ranging from 0.07 mm to 0.19 mm. growth was observed on molybdenum-deficient N-free medium for Azotobacter and Beijerinckia only. Growth was observed in peptone agar for Azotobacter and Azomonas only. Genomic DNA was extracted using boiling-lysis and phenol-chloroform extraction. Cells for extraction were either pelleted from liquid medium or obtained directly from colonies on agar plates. PCR detection of nijH gene was initially performed to verify nitrogen-fixing capability of rhizobacterial isolates. However, no PCR products were obtained. The nijH gene may be found on the plasmid DNA. This could also be attributed to the PCR conditions that lack optimization. This is the first study on isolation and identification of nitrogen-fixing rhizobacterial in the sago palm in Mindanao
| Item type | Current library | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Thesis | University Library | Non-Circulation | LG993.5 2005 B4 O84 (Browse shelf(Opens below)) | Available | 3UPML00022055 | |
| Thesis | University Library | Reference/Room-Use Only | LG993.5 2005 B4 O84 (Browse shelf(Opens below)) | Available | 3UPML00011355 |
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Thesis (BS Biology) -- University of the Philippines Mindanao, 2005
Three bacterial isolates were obtained from soil samples taken from Simulao, Bunawan (SI), a ricefield water-logged for most of the year, and Pulangpula, Trento (PL), found 1.3m from the bank of a freshwater stream. Azotobacter and Azomonas-specific medium and Beijerinckia-specific medium were used. Growth of the isolates was poorer on Beijerinckia-specific medium compared to that on Azotobacter and Azomonas-specific medium. Using conventional microbiological methods, the identity of each isolate was determined. PL-A1, identified to be Azotobacter, were Gram-positive, motile, aerobic, and catalase coccoids ranging from 1.5m to 2.5m in diametrr. Cysts formed ranged from 1.5m to 3.0m in diameter. Colonies formed were cream in color, circular, entire, convex, mucoid, and glistening, with diameter ranging from 0.01 mm to 0.16 mm. PL-A2, identified as Beijerinckia, were Gram-negative. Motile, aerobic, and catalase-positive slightly curved rods with the dimensions 0.5-1.5 m x 1.5-2.5 m. colonies formed were transparent, entire, convex, mucoidal and slimy measuring 0.08 mm to 0.51 mm in diameter. SI-B, identified as Azomonas, were Gram-negative, motile, aerobic, and catalese-positive coccoids ranging from 1.5 m to 3.0 m in diameter. Colonies formed were cream in color, circular, entire, convex, mucoid and glistening, with diameter ranging from 0.07 mm to 0.19 mm. growth was observed on molybdenum-deficient N-free medium for Azotobacter and Beijerinckia only. Growth was observed in peptone agar for Azotobacter and Azomonas only. Genomic DNA was extracted using boiling-lysis and phenol-chloroform extraction. Cells for extraction were either pelleted from liquid medium or obtained directly from colonies on agar plates. PCR detection of nijH gene was initially performed to verify nitrogen-fixing capability of rhizobacterial isolates. However, no PCR products were obtained. The nijH gene may be found on the plasmid DNA. This could also be attributed to the PCR conditions that lack optimization. This is the first study on isolation and identification of nitrogen-fixing rhizobacterial in the sago palm in Mindanao
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