Optimization of a Microwell cytotoxicity assay using Artemia salina (brine shrimps) /
Myra Sharon Malicsi
- 2000
- 25 leaves
Thesis (BS Biology) -- University of the Philippines Mindanao, 2000
The microwell cytotoxicity assay using Artemia salina (brine shrimps) was done as follows: (1) Artemia salina cysts, 0.25 grams, was hatched in 100 ml artificially prepared seawater supplemented with 0.006 grams of yeast extract, (2) continuous moderate aeration was provided in the culture media, (3) serial dilutions of chloramphenicol, cyclohexanone and vincristine were made in the well of the 96-microwell plate, (4) a suspension containing 10-15 nauplii was added tp each well. (5) after 24 hours of hydration, the number of dead nauplii per well was counted, and (6) media lethal concentration was determined using the Probit analysis. It was found that efficiency and rate of hatching increased with salt concentration in the culture media. The 24 hr. LC50 of chloramphenicol, cyclohexanone and vincristine were 39450.12 mg/ml, 5007.907 mg/ml and 1023.712 mg/ml, respectively