TY  - BOOK
AU  - Bullo, Lani Lee Rigor.
TI  - Production studies, purification,and characterization of amylolytic enzyme from saccharomycopsis fibuligera 2074 isolated from Philippine bubod starter
PY  - 2009///
KW  - Amylases
KW  - Bubod yeasts
KW  - Amylase isolation
KW  - Enzyme characterization
KW  - Enzyme production
KW  - Protein purification
KW  - Saccharomycopsis fubuligera 2074
KW  - Starch-digesting amylase
KW  - Hydrolysis
KW  - Starch
KW  - Undergraduate Thesis
KW  - FST200,
KW  - BSFT
N1  - Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2009
N2  - Saccharomycopsis fibuligera 2074 was identified to produce the highest extracellular raw starch-digesting amylase among eight amylolytic bubod isolates screened. Maximum growth and amylase production was obtained from 36-hour culture using 1% raw sago starch as the major carbon source. The enzyme was purified 2.56- fold, with a specific activity of 180.49 U/mg after DEAE-cellulose anion-exchange column chromatography. Amylase activity was optimum at pH of 6 and at 400C, Although the enzyme was inhibited by CU2+, Zn2+ and Al3+, it was activated by Ca2+, Fe3+, Ba2+, phenylmethylsulfonyl fluoride and ethylenediaminetetraacetic acid. On the other hand, iodoacetic acid K+, Cd2+, and Mg2+, showed no significant effect on amylase activity. The purified amylase showed interesting properties necessary for industrial application
ER  -