TY  - BOOK
AU  - Cancio,Leslie pearl Mansilita
TI  - Partial purification and characterization of activity peak B amylolytic enzyme from Saccharomycopsis bubodii 2066
PY  - 2009///
KW  - Undergraduate Thesis
KW  - FST200,
KW  - BSFT
N1  - Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2009
N2  - Extracellular amylolytic enzyme production of Saccharomycopsis bubodii 2066 was enhanced using raw sago starch as carbon source and incubating the culture for 9 h without agitation. After DEAE-cellulose column chromatography, two activity peaks were observed. Activity peak B, obtaining the highest specific activity of 46.57 U/mg and was partially purified by 2.02 fold, was used for further characterization. Optimum activity of the enzyme was observed at 400C and pH 4.5. maximum stability was obtained at 30-400C and pH range of 4.0-5.0. the enzyme was activated by Ca2+ and was inactivated by Ba2+, Cu2+, Mg2+, Zn2+ and K+. other chemical modifiers like Cd2+, Al3+, Fe3+, EDTA and iodoacetic acid showed no significant effect on the activity of the enzyme
ER  -