Characterization of three potential plant growth-promoting rhizobacteria from sago palm (metroxylon sagu rottb.)/ Angealyn A. Gealon.

By: Material type: TextTextLanguage: English Publication details: 2008Description: 91 leavesSubject(s): Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2009 Summary: Three potential plant growth-promoting rhizobacteria designated as Rh1-MS-CO, Rh2-MS-CO and Rh3-MS-CO showed characteristics of the family Azotobacteriaceae. Phenotypic charateristics were made in in vitro assay to identify the isolates down to the species level. Morphological, biochemical tests and growth features in culture media such as their carbon and nitrogen- source utilization as bases of description for each isolate. Results identified the strains Rh1-MS-CO, Rh2-MS-CO and Rh3-MS-CO possibly as Azomonas macrocytogenes, Beijerinckia mobilis and Gluconacetobacter diazotrophicus, respectively. They were Gram negative, motile, strict aerobes, and grew in Dobereiner N-free medium, suggesting the ability of these isolates to facilitate microaerophilic nitrogen fixation. Isolates in carbon-nitrogen sources, glucose and NH4Cl ably supported the growth of the isolates. Glucose and sucrose provided the best sources of carbon as compared with mannitol and ethanol. None of the aminoacids were utilized as nitrogen source. Peptone concentrations ranging from 0.05 to 1.0% w/v under continuous shaking conditions provided the optimum growth for the three isolates. Significant growth increases were recorded for peptone concentrations to affect growth counts for A. macrocytogenes and B. mobilis. Both variables of shaking and peptone concentrations were able to affect G. diazotrophicus growth count. Increasing color changes were significantly observed n B. mobilis and G diazotrophics as peptone concentrations increased. Results were positive in the indole test for all three isolates, supporting their potential as agents of bacterial growth. This was a strong indication of their potential as biofertilizers
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Item type Current library Collection Call number Status Date due Barcode
Thesis University Library Non-Circulation LG993.5 2008 B4 G43 (Browse shelf(Opens below)) Available 3UPML00032430
Thesis University Library Reference/Room-Use Only LG993.5 2008 B4 G43 (Browse shelf(Opens below)) Available 3UPML00012365

Thesis (BS Biology) -- University of the Philippines Mindanao, 2009

Three potential plant growth-promoting rhizobacteria designated as Rh1-MS-CO, Rh2-MS-CO and Rh3-MS-CO showed characteristics of the family Azotobacteriaceae. Phenotypic charateristics were made in in vitro assay to identify the isolates down to the species level. Morphological, biochemical tests and growth features in culture media such as their carbon and nitrogen- source utilization as bases of description for each isolate. Results identified the strains Rh1-MS-CO, Rh2-MS-CO and Rh3-MS-CO possibly as Azomonas macrocytogenes, Beijerinckia mobilis and Gluconacetobacter diazotrophicus, respectively. They were Gram negative, motile, strict aerobes, and grew in Dobereiner N-free medium, suggesting the ability of these isolates to facilitate microaerophilic nitrogen fixation. Isolates in carbon-nitrogen sources, glucose and NH4Cl ably supported the growth of the isolates. Glucose and sucrose provided the best sources of carbon as compared with mannitol and ethanol. None of the aminoacids were utilized as nitrogen source. Peptone concentrations ranging from 0.05 to 1.0% w/v under continuous shaking conditions provided the optimum growth for the three isolates. Significant growth increases were recorded for peptone concentrations to affect growth counts for A. macrocytogenes and B. mobilis. Both variables of shaking and peptone concentrations were able to affect G. diazotrophicus growth count. Increasing color changes were significantly observed n B. mobilis and G diazotrophics as peptone concentrations increased. Results were positive in the indole test for all three isolates, supporting their potential as agents of bacterial growth. This was a strong indication of their potential as biofertilizers

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