Due to potential and present ecological alteration in roosting sites and food source areas in neighboring vicinity of Caraga State University in Caraga Region, Philippines, unsettled progression of bats are documented through wing variation analysis and fluctuating asymmetry (FA). Twenty-one (21) collected samples of Ptenochirus jagori (Greater Musky Fruit Bat) yields asymmetry of 90.6396%, affecting landmarks of the elbow (landmark 1), carpals (landmark 2), metacarpals (landmark 3, 4 and 10), and phalanges' fingers (landmarks 5, 6, 11, and 12). Wing variation (landmark-based) analysis suggested significant skewness on both elbows and metacarpals of wings. Wider shape deformation is on the right-wing, and a narrower one was analyzed on the left. Nonetheless, even though similar alteration is present between both wings, Canonical Variation Analysis (CVA) suggests no significant difference in the deformations of histogram analysis.