000			02062nam a22003613a 4500
001			UPMIN-00003300472
003			UPMIN
005			20240525153428.0
008			240525b |||||||| |||| 00| 0 eng d
040			_aDLC _cUPMin _dupmin
041			_aeng
090		0	_aLG993.5 2008 _bF62 T32
100			_aTabanao, Glaiza Lumabi. _eauthor _925895
245			_aAmylase production studies and partial purification of amylolytic enzyme from Enterococcus Faecium DMF78 / _cGlaiza L. Tabanao
260			_c2008
300			_a45 leaves.
502			_aThesis (BS Food Technology) -- University of the Philippines Mindanao, 2008
520	3		_aThe amylolytic enzyme of Enterococcus faecium DMF78 was isolated and partially purified from the fermentation broth. Amylase production and enzyme assay conditions were optimized prior to purification. Amylase production was found to be better at the following cultural conditions: raw sago starch as carbon source and incubation without shaking. The highest productivity of amylases was obtained at the first hour of incubation and generally declined thereafter. Prior to the purification protocol was sequenced as follows: ammonium sulfate precipitation at 90% saturation, dialysis and anion-exchange chromatography (DEAE-cellulose). The partially purified amylase preparation had a purification-fold of 1.57, a recovery of 24.69% and specific activity of 115.31 U/mg. Purity was monitored using SDS-PAGE analysis but distinct protein bands were not observed probably due to very dilute samples.
650	1	7	_aAmylase enzymes. _925896
650	1	7	_aAmylase _xProduction. _925897
650	1	7	_aStarch. _91680
650	1	7	_aAlpha-amylase. _925898
650	1	7	_abeta-amylase. _925899
650	1	7	_aPullulanase. _925900
650	1	7	_aCycloderxtrin glycosyltransferase. _925901
650	1	7	_aAmyloglucosidase _925902
650	1	7	_aEnterococcus faecium. _925232
650	1	7	_aCrude enzyme extraction. _925903
658			_aUndergraduate Thesis _cFST200, _2BSFT
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c2291 _d2291