000			02028nam a22003613a 4500
001			UPMIN-00003300492
003			UPMIN
005			20230126164550.0
008			230126b |||||||| |||| 00| 0 eng d
040			_aDLC _cDLC _dupmin
041			_aeng
090		0	_aLG993.5 2009 _bF62 B84
100			_aBullo, Lani Lee Rigor. _91670
245			_aProduction studies, purification,and characterization of amylolytic enzyme from saccharomycopsis fibuligera 2074 isolated from Philippine bubod starter / _cLani Lee Rigor Bullo.
260			_c2009
300			_a73 leaves.
502			_aThesis (BS Food Technology) -- University of the Philippines Mindanao, 2009
520	3		_aSaccharomycopsis fibuligera 2074 was identified to produce the highest extracellular raw starch-digesting amylase among eight amylolytic bubod isolates screened. Maximum growth and amylase production was obtained from 36-hour culture using 1% raw sago starch as the major carbon source. The enzyme was purified 2.56- fold, with a specific activity of 180.49 U/mg after DEAE-cellulose anion-exchange column chromatography. Amylase activity was optimum at pH of 6 and at 400C, Although the enzyme was inhibited by CU2+, Zn2+ and Al3+, it was activated by Ca2+, Fe3+, Ba2+, phenylmethylsulfonyl fluoride and ethylenediaminetetraacetic acid. On the other hand, iodoacetic acid K+, Cd2+, and Mg2+, showed no significant effect on amylase activity. The purified amylase showed interesting properties necessary for industrial application.
650	1	7	_aAmylases. _91671
650	1	7	_aBubod yeasts. _91672
650	1	7	_aAmylase isolation. _91673
650	1	7	_aEnzyme characterization. _91674
650	1	7	_aEnzyme production. _91675
650	1	7	_aProtein purification. _91676
650	1	7	_aSaccharomycopsis fubuligera 2074. _91677
650	1	7	_aStarch-digesting amylase. _91678
650	1	7	_aHydrolysis. _91679
650	1	7	_aStarch. _91680
658			_aUndergraduate Thesis _cFST200, _2BSFT
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c2301 _d2301