000			02067nam a2200241 4500
001			UPMIN-00004099664
003			UPMIN
005			20240528100959.0
008			100129s2009 ph eng
040			_aDLC _cUPMin _dupmin
041			_aeng
090		0	_aLG993.5 2009 _bF62 T57
100	1		_aTirol, Ara Mae Magno _eauthor _925919
245	0	0	_aMembrane solubilization of porcine pulmonary and renal angiotensin I-converting enzyme (ACE; E.C. 3.4.15.1) / _cAra Mae Magno Tirol
260			_c2009
300			_a41 leaves
502			_aThesis (BS Food Technology) -- University of the Philippines Mindanao, 2009
520	3		_aPulmonary and renal porcine ACE (angiotensin I-converting enzyme) was isolated from the lung and kidney of pig which was obtained from the slaughterhouse in Mintal, Davao City. The tissues from lung and kidney were solubilized using four different procedures after which the activities were measured by spectrophotometric method in the presence of quinolone and benzenesulfonyl chloride (BSC). The initial ACE activity from lung and kidney extract was 0.0409 U/mg and 0.0788 U/mg, respectively. The first method was by incubation with shaking at room temperature which resulted to an activity of 0.0393 U/mg for lungs and 0.1008 U/mg for kidney. The second procedure which used sonication to solubilize the membranes resulted to activities of 0.0390 U/mg for lungs and 0.0828 U/mg for kidneys. The third procedure was the addition of EDTA with the presence of Triton X-100 which decreased the activities to ? 0.0019 U/mg for lungs and 0.0015 U/mg for kidneys. The last procedure was the addition of the detergent using Triton X-100. The activity was reported to have increased to 0.1639 U/mg for lung and 0.3374 U/mg for kidney ACE. From the results of the four solubilization procedures the best procedure was the employment of Triton X- 100 which significantly increased the activities for both lung and kidney ACE.
658			_aUndergraduate Thesis _cFST200, _2BSFT
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c2322 _d2322