000			02170nam a22002893a 4500
001			UPMIN-00005017247
003			UPMIN
005			20240228160451.0
008			240228b |||||||| |||| 00| 0 eng d
040			_aDLC _cUPMin _dupmin
041			_aeng
090		0	_aLG993.5 2010 _bF62 G86
100			_aGumia, Robelyn Pieza. _eauthor _925325
245			_aIsolation and partial characterization of a potential amylolytic microorganism from decomposing sago palm pith / _cRubelyn Pieza Gumia.
260			_c2010
300			_a55 leaves
502			_aThesis (BS Food Technology) -- University of the Philippines Mindanao, 2010
520	3		_aSixty-seven potentially amylolytic microorganisms were isolated from decomposing sago palm pith. Twenty-five starch hydrolyzing isolates were purified and screened for amylolytic activity. SB125, the most promising isolate, had an amylolytic activity of 1.45 U/mL. Morphological biochemical characterization of SB 125 revealed that it is a coccus, facultatively aerobic and Gram-negative. It is tested negative for the catalase test, for indole production and litmus milk reaction. However, it was positive for methyl red staining and starch hydrolysis. Amylase activity increased when SB 125 was grown on modified de Mann, Rogosa and Sharpe (MRS) medium without Ca2+ supplementation, under shaking condition, Partial enzyme purification yielded a specific activity of 5.51 U/mg at 1.32 fold purification and 13.4% recovery. The partially purified enzyme had an optimum activity at 400C and pH 8.5. enzyme immobilized by surface adsorption yielded a specific activity of 11.87 U/mg, significantly higher than the unimmobilized enzyme which yielded 5.51 U/mg. Enzyme immobilized by entrapment showed a decrease in activity at 3.65 U/mg, indicating that surface adsorption is more promising if SB 125 were to be developed for industrial application.
650	1	7	_aAmylase _925326
650	1	7	_aRotting sago palm pith _925327
650	1	7	_aEnzyme characterization _91674
650	1	7	_aEnzyme immobilization _925328
658			_aUndergraduate Thesis _cFST200, _2BSFT
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c2484 _d2484