000			02344nam a22003253a 4500
001			UPMIN-00005875637
003			UPMIN
005			20240525124044.0
008			240525b |||||||| |||| 00| 0 eng d
040			_aDLC _cUPMin _dupmin
041			_aeng
090		0	_aLG 993.5 2011 _bF62 S56
100			_aSimogan, Jerry P., Jr. _eauthor _925878
245			_aExtraction and partial purification of proanthocyanidins (PAC) from cacao seeds / _cJerry Parparan Simogan, Jr.
260			_c2011
300			_a44 leaves.
502			_aThesis (BS Food Technology) -- University of the Philippines Mindanao, 2011
520	3		_aExtraction from fresh cacao seeds was carried out using 25%, 50%, 70%, and 95%n ethanol to determine what concentration gives the highest yield in extracting proanhocyanidins (PAC). The trend showed that as ethanol concentration increased, the yield also increased (25%=20.67%, 50%-50.80%, 70%-58.47% and 95%=80.20% of the entire PAC content of cacao seeds). The 95% EtOH, which yielded the highest, was then used for assessing efficiency of extraction protocol employed. The triple extraction test showed that single extraction is enough to extract majority of the PAC of the cacao seed powder and that 95% EtOH is far better solvent than hot water to extract PAC. The 95% EtOH was used for extracting crude PAC which was purified alter using Diaion HP-20 resin chromatographic column. There was a total yield from the water and 10% EtOH washing of 38.519% monitored through OD 280 scanning, which constitutes the contaminants removed from the extract. Proanthocyanidins were eluted using 70% ethanol. The chromatographic procedure using the Diaion enriched the PAC content per unit absorbance at 280 nm (OD280) of the crude extract 1.62 fold; thus, a partially purified PAC extract was obtained. There was an excellent recovery of the PAC content of the crude extract and no loss was recorded after purification.
650	1	7	_aProanthocyanidins. _925879
650	1	7	_aExtraction. _925880
650	1	7	_a95% ethanol. _925881
650	1	7	_aPurification by Dia-ion HP20 _925882
650	1	7	_aCacao seeds. _925883
650	1	7	_aPAC (Proanthocynidins) _925884
650	1	7	_aCrude extract. _925885
658			_aUndergraduate Thesis _cFST200, _2BSFT
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c2603 _d2603