000			02188nam a22003133a 4500
001			UPMIN-00005875638
003			UPMIN
005			20240227142841.0
008			230130b |||||||| |||| 00| 0 eng d
040			_aDLC _cUPMin _dupmin
041			_aeng
090		0	_aLG 993.5 2011 _bF62 C38
100			_aCaubalejo, Rey Jacutin. _91750 _eauthor
245			_aCharacterization of hydrolytic activity of raw starch-digesting amylase (RSDA) from Saccharomycopsis bubodii 2066 on sago (Metroxylon sagu Rottb.) starch / _cRey Jacutin Caubalejo.
260			_c2011
300			_a76 leaves.
502			_aThesis (BS Food Technology) -- University of the Philippines Mindanao, 2011
520	3		_aA raw starch digesting amylase (RSDA) was purified from Saccharomycopsis bubodii 2066 which was isolated from Philippine Bubod starter. The free enzyme (dialysate) had a specific activity of 3.91U mg-1. It was immobilized by encapsulation with calcium alginate. Specific activity of the immobilized enzyme decreased to 0.26 U mg-1. Hydrolitic activity was evaluated through degree and rate of hydrolysis by determining reducing sugar content as a function of time. The reaction mixtures contained 0.11 mg protein and 0.7 mg protein, for the free and immobilized enzymes, respectively. Degree of hydrolysis using free and immobilized enzyme was 0.11 and 0.03 mg reducing sugar mg-1 protein, respectively. Adsorbability onto raw sago starch was not observed in immobilized enzyme which explains the negative results. On the contrary, the free enzyme almost adsorbed completely. Operational stability in immobilized enzyme was assessed through binding efficiency and enzyme reusability. Binding efficiency was 70.75% efficiency. After reusing the bead for 4 cycles. it retained 18.24% of the original activity.
650	1	7	_aRSDA (Raw Starch Digesting Amylase) _91751
650	1	7	_aSaccharomycopsis bubodii 2066 _91752
650	1	7	_aHydrolytic activity. _91753
650	1	7	_aImmobilized enzymes. _91754
650	1	7	_aGlucoamylase. _91755
650	1	7	_aSago starch. _91454
658			_aUndergraduate Thesis _cFST200, _2BSFT
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c2604 _d2604