000 | 01935nam a2200205 4500 | ||
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001 | UPMIN-00000009309 | ||
005 | 20220921142333.0 | ||
040 |
_aDLC _cDLC _dupmin |
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041 | _aeng | ||
090 |
_aLG993.5 2003 _bB4 M37 |
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100 | 1 | _aMaquilan, Mary Ann D. | |
245 | 0 | 0 |
_aInitial studies on in vitro germination and early seedling growth of Nepenthes truncata MACF / _cMary Ann D. Maquilan |
502 | _aThesis (BS Biology) -- University of the Philippines Mindanao, 2003 | ||
300 | _a71 leaves | ||
260 | _c2003 | ||
520 | _aA protocol for in vitro seed germination of the endangered Philippine pitcher plant, Nepenthes truncate, was developed from a series of seed sterilization trials and germination experiments involving three factors: seed pretreatments, culture medium and salt concentration. Germination studies were done in independent but simultaneous experiments using flasks and jars. Highest efficiency of seed sterilization (90.8%) was obtained by a combination of 70% isopropyl alcohol, 10% commercial bleach, and 3%hydrogen peroxide. This procedure resulted in 83.7% germination based on a sample size of 520 cultures. Germination in in vitro culture was achieved in 18 to 74 days. A previous study reported germination time of 28 to 180 days. Soaking in sugar solution could increase the initial germination percentage. The treatment that gave the highest final germination percentage was a combination of seed hardening and Murashige and Skoog (92.4%) or Knudson C medium (92.2%). Flasks capped with paper and aluminum sheets produced healthier seedlings than jars with unvented metal caps. Knudson C gave superior effects on seedlings that eventually became chlorotic of necrotic and were not able to supervise longer in cultures. Abnormalities were most severe in seedlings exposed to three-fourth to full concentration of MS salts. | ||
658 |
_aUndergraduate Thesis _cBIO200, _2BSB |
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905 | _aFi | ||
905 | _aUP | ||
999 |
_c335 _d335 |