000			02240nam a2200325 4500
001			UPMIN-00000014743
005			20220921142406.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2007 _bB4 B66
100	1		_aBongcawil, Roxanne Yen C.
245	0	0	_aIn vitro morphological studies and cultural characterization of Pestalotiopsis Sp. associated with Nepenthes truncata macf. / _cRoxanne Yen C. Bongcawil.
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2007
300			_a40 leaves
650	0		_aGungi.
650			_aNepenthes species.
650			_aFungal diseases.
650			_aPathogenicity.
650			_aInoculum.
650			_aInoculation.
650			_aReisolation.
650			_aVitro.
650			_aMorphological _xStudies.
650			_aGermination.
260			_c2007
520			_aA fungus pestalotiopsis sp. from brown leafspot-infected pitcher plants (Nephentes truncate macf.) in Marilog District, Davao City was isolated to pure culture. Fungus growth in plated Potato Dextrose Agar (PDA) appeared white and cottony with punctiform acervuli. Under microscopy, the fungus hypha is hyaline, septated with hyphal cells measuring 23.99 (1.40)m wide. The conidia, which define the genius, are fusiform with 3 yellowish-brown median cells and hyaline appendages at opposite ends. The basal portion having 1 and the apical portion with 2 to 3 appendages. Total spore size is 22.09 ((1.73)m long and 6.11 (0.64)m wide. The 3 median cells measured 15 (1.17)m long. The basal appendage is 5.04 (1.69)m long while the apical appendages ranged from 9.54 to 34.09m, with an average of 20.41 (6.35)m ling. In axenic PDA medium, cultures grown under alternating 12-hr light and 12-hr darkness had the widest colonies, however, spore production was highest with continuous light after a 7-day growth time. No spore was observed on cultures under continuous darkness. Colony growth was widest and spore production highest with cultures under pH 8. Mycelial growth was not significantly influenced by culture temperatures, 230C and 27oC, however, spore production was higher in the former
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
999			_c681 _d681