Membrane solubilization of porcine pulmonary and renal angiotensin I-converting enzyme (ACE; E.C. 3.4.15.1) / (Record no. 2322)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02067nam a2200241 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00004099664 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | UPMIN |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240528100959.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100129s2009 ph eng |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | UPMin |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 #0 - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2009 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | F62 T57 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Tirol, Ara Mae Magno |
| Relator term | author |
| 9 (RLIN) | 25919 |
| 245 00 - TITLE STATEMENT | |
| Title | Membrane solubilization of porcine pulmonary and renal angiotensin I-converting enzyme (ACE; E.C. 3.4.15.1) / |
| Statement of responsibility, etc. | Ara Mae Magno Tirol |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2009 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 41 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Food Technology) -- University of the Philippines Mindanao, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Pulmonary and renal porcine ACE (angiotensin I-converting enzyme) was isolated from the lung and kidney of pig which was obtained from the slaughterhouse in Mintal, Davao City. The tissues from lung and kidney were solubilized using four different procedures after which the activities were measured by spectrophotometric method in the presence of quinolone and benzenesulfonyl chloride (BSC). The initial ACE activity from lung and kidney extract was 0.0409 U/mg and 0.0788 U/mg, respectively. The first method was by incubation with shaking at room temperature which resulted to an activity of 0.0393 U/mg for lungs and 0.1008 U/mg for kidney. The second procedure which used sonication to solubilize the membranes resulted to activities of 0.0390 U/mg for lungs and 0.0828 U/mg for kidneys. The third procedure was the addition of EDTA with the presence of Triton X-100 which decreased the activities to ? 0.0019 U/mg for lungs and 0.0015 U/mg for kidneys. The last procedure was the addition of the detergent using Triton X-100. The activity was reported to have increased to 0.1639 U/mg for lung and 0.3374 U/mg for kidney ACE. From the results of the four solubilization procedures the best procedure was the employment of Triton X- 100 which significantly increased the activities for both lung and kidney ACE. |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | FST200, |
| Source of term or code | BSFT |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Library of Congress Classification |
| Koha item type | Thesis |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Status | Collection | Home library | Current library | Shelving location | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Library of Congress Classification | Preservation Copy | Non-Circulating | University Library | University Library | Archives and Records | 2022-10-05 | UAR-T-gd1499 | LG993.5 2009 F62 T57 | 3UPML00033171 | 2022-10-05 | 2022-10-05 | Thesis | ||||
| Library of Congress Classification | Room-Use Only | College of Science and Mathematics | University Library | Theses | 2022-10-05 | CSM-T-gd2223 | LG993.5 2009 F62 T57 | 3UPML00012499 | 2022-10-05 | 2022-10-05 | Thesis |